TriLink BioTechnologies

TriLink BioTechnologies is a specialized CDMO focused on nucleic acid manufacturing and mRNA technologies. The company offers:

mRNA Manufacturing & Capping:

• Proprietary CleanScript™ in vitro transcription method optimized to reduce dsRNA
• CleanCap® one-pot capping solutions generating Cap1 structure with >95% efficiency
• mRNA synthesis scales from milligram to multi-gram quantities
• Construct lengths from hundreds of nucleotides to over 14 kilobases

Oligonucleotide Synthesis:

• DNA and RNA oligonucleotides at scales up to multiple grams
• Specialized synthesis of challenging constructs including gRNA, siRNA, microRNA, and aptamers
• Extensive modification options: backbone modifications, dye labels, biotin conjugation, linkers, and spacers
• Industry-leading quality control via PAGE, HPLC, and MS

Custom Nucleotides:

• Synthesis of ultrapure novel nucleotides and modified amidites
• Over 150 modified nucleoside triphosphates (NTPs) in portfolio
• Custom chemistry from discovery phase through multi-kilogram scale-up
• Specialty modified nucleotides including 5'-3' bisphosphates, monophosphates, and alpha-thiol NTPs

Analytical & QC Services:

• In-house method development and analytical testing capabilities
• Identity, characterization, purity, and impurity analysis
• Safety testing including contaminating nuclease detection for GMP products
• Construct-specific assays and QC support across all product lines

Infrastructure:

• 118,000 sq ft facility with multiple specialized manufacturing suites and support labs
• Scalable processes designed to maintain timelines and accommodate demand surges
• GMP-grade manufacturing capabilities